Endophytic fungi from Camellia sinensis show an antimicrobial activity against the rice blast pathogen Magnaporthe grisea;
Hongos endofíticos de Camellia sinensis muestran una actividad antimicrobiana contra el tizón del arroz Magnaporthe grisea
These authors contributed equally to this work., Hu, YF; Tea Science Research Institute, Nanjing Agricultural University, Nanjing 210095, P.R. China.
† These authors contributed equally to this work., Chen, X; Tea Science Research Institute, Nanjing Agricultural University, Nanjing 210095, P.R. China., Wang, YH; Tea Science Research Institute, Nanjing Agricultural University, Nanjing 210095, P.R. China., Fang, WP; Tea Science Research Institute, Nanjing Agricultural University, Nanjing 210095, P.R. China., Li, XH; Tea Science Research Institute, Nanjing Agricultural University, Nanjing 210095, P.R. China.
The purpose of this study was to evaluate the antagonistic activity of two endophytic fungal strains, Pseudocercospora kaki and Penicillium sclerotiorum, isolated from the leaves of Camellia sinensis, against the rice blast pathogen Magnaporthe grisea. The inhibitory activity of the two endophytes against M. grisea in dual-culture was compared with that in monoculture. It was confirmed that the broth and its ethyl acetate extract of the dual-culture had a much stronger inhibition activity against M. grisea than the monocultures of P. kaki and P. sclerotiorum. The antagonism index of the broth and ethyl acetate extract from dual-culture to the mycelial growth of M.grisea was 78.02% ± 2.19% and 62.81% ± 2.29%, respectively, in different incubation periods. Qualitative analysis of ethyl acetate extractby GC-MS revealed that the number of bioactive compounds was greater in dual-culture than in monoculture. Compared to the ethyl acetate extract from monoculture, there were 10 constituents of bioactive compounds from dual-culture; however, there were only 6 types and 5 types from P. kaki monoculture and P. sclerotiorum monoculture, respectively. Glycerol; 4-Hydroxyphenyl ethanol; 1,2,3,4-Tetrahydroxy valeraldehyde and 1,2-Benzene-dicarboxylic acid dicyclohexyl ester were found in the ethyl acetate extracts of P. kaki, P. sclerotiorum and the dual culture. Based on the composition of the ethyl acetate extract of the dual culture of the endophytic fungi it might be possible to make a bioformulation for the biocontrol of the plant pathogen.
El propósito de este estudio fue evaluar la actividad antagonista de dos cepas de hongos endofíticos, Pseudocercospora kaki y Penicillium sclerotiorum, aislados de las hojas de Camellia sinensis, en relación al patógeno del tizón del arroz Magnaporthe grisea. La actividad inhibitoria de las dos cepas endófitas sobre M. grisea en cultivo dual se comparó con aquella en monocultivo. Se confirmó que el caldo y su extracto de etil acetato del cultivo dual tuvieron una actividad mucho más inhibitoria sobre M. grisea que los monocultivos de P. kaki y P. sclerotiorum. Los índices antagonistas del caldo y extracto de etil acetato del cultivo dual sobre el crecimiento del micelio de M. grisea fueron 78,02% ± 2,19% y 62,81% ± 2,29%, respectivamente, en diferentes períodos de incubación. El análisis cualitativo del extracto de etil acetato por GC-MS reveló que el número de compuestos bioactivos fue mayor en el cultivo dual que en el monocultivo. Comparado al extracto de etil acetato del monocultivo, hubo 10 compuestos bioactivos en el cultivo dual; sin embargo, hubo solo 6 tipos en el monocultivo de P. kaki, y 5 tipos en el monocultivo de P. sclerotiorum. Glicerol; 4-Hidroxifenil etanol; 1,2,3,4-tetrahidroxi valeraldehido y ester biciclohexil 1,2-benceno-ácido bicarboxílico se hallaron en los extractos de etil acetato de P. kaki, P. sclerotiorum y el cultivo dual. Basado en la composición del extracto de etil acetato del cultivo dual del hongo endofítico se podría hacer una formulación biológica para el control biológico del patógeno vegetal.
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These authors contributed equally to this work.
† These authors contributed equally to this work.