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Comunicación - Archivo PPCT

Determinación de apoptosis por TUNEL e inmunoreactividad de las proteínas BCL-2 y BAX durante la placentación porcina. Comunicación

Creators:Cristofolini, A.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba, Merkis, A.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba, Barroso, F.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba, Vaquer Balmaceda, V.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba, Allende, F.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba, Chanique, A.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba, Koncurat, M.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba
2013-05-24

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Descripción

El objetivo del presente trabajo fue estudiar en muestras placentarias porcinas de diferentes estadios gestacionales, la fragmentación del ADN a través de la técnica de TUNEL y la inmunoreactividad de las proteínas reguladoras BAX y BCL-2, a fin de detectar los mecanismos pro y antiapoptóticos de la vía mitocondrial involucrados en la remodelación celular placentaria. Se utilizaron cortes histológicos de ± 4 μm provenientes de placentas porcinas de ± 30, 55, 70 y 114 días de preñez. Para la detección de las proteínas BAX y BCL-2 se realizó la técnica de inmunoperoxidasa utilizando anticuerpos comerciales específicos y para la detección de la fragmentación de ADN se utilizó la técnica de TUNEL. Los resultados se expresaron en forma semicuantitativa y para cada período gestacional se determinó la distribución de la intensidad de inmunomarcación a través del valor de High Score (HS). En placentas de 30 días de preñez se observaron núcleos apoptóticos característicos distribuidos en forma de parches en el epitelio materno, mientras que la marcación se presentó en forma lineal en los demás períodos placentarios. En los diferentes períodos gestacionales la proteína proapoptótica BAX se encuentra regulada por la expresión del marcador antiapoptótico BCL-2. Al inicio de la gestación se detectaron valores de HS positivos con el marcador apoptótico BAX, los mismos aumentaron hacia los días 55 y 114 de preñez y disminuyeron hacia el día 70 de gestación. En conclusión, al inicio de la gestación porcina la remodelación placentaria observada acontece por inducción de la vía intrínseca a través de BAX, siendo mayor hacia los 55 y 114 días de preñez.

Metadatos destacados

Colecciones
Revista Argentina de Producción Animal

Editor

Asociación Argentina de Producción Animal

Fuente

Revista Argentina de Producción Animal; Vol 30, No 1 (2010): Enero - Junio 2010; 43-49

Citación

Cristofolini, A.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba et al., “Determinación de apoptosis por TUNEL e inmunoreactividad de las proteínas BCL-2 y BAX durante la placentación porcina. Comunicación,” Archivo PPCT, consulta 4 de abril de 2026, http://archivoppct.caicyt.gov.ar/items/show/8629.

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Materia

Autor

Cristofolini, A.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba
Merkis, A.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba
Barroso, F.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba
Vaquer Balmaceda, V.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba
Allende, F.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba
Chanique, A.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba
Koncurat, M.; Facultad de Agronomía y Veterinaria. Universidad Nacional de Río Cuarto. Río Cuarto. Córdoba

Fuente

Revista Argentina de Producción Animal; Vol 30, No 1 (2010): Enero - Junio 2010; 43-49

Editor

Asociación Argentina de Producción Animal

Fecha

2013-05-24

Formato

Idioma

spa

Tipo

info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion